Translational_Unit

Part:BBa_K906100:Design

Designed by: Lucas B. Harrington   Group: iGEM12_WashU   (2012-08-13)


Extended psbA2 with Zeaxanthin 7,8-cleavage dioxygenase (ZCD)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 974
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The point mutation (A->T) enables the use of NdeI as mentioned on the main page.


Source

psbA2 is from the genomic sequence preceding the photosystem II D1 protein coding region in Synechocystis spp. 6803. The modified version of ZCD has been codon optimized for the species Synechocystis 6803.

References